Provided by: drop-seq-tools_3.0.2+dfsg-1_all 

NAME
create_Drop-seq_reference_metadata.sh - create Drop-seq reference metadata bundle
DESCRIPTION
USAGE: create_Drop-seq_reference_metadata.sh [options]
OPTIONS
-n <name> Name for reference metadata set to be created. Required.
-r <referencefasta>
Reference fasta of the Drop-seq reference metadata bundle. Required.
-s <species>
Species. Required.
-g <gtf>
Gene annotation file. Required.
-f <filtered-gene-biotype>
Annotations with the given gene_biotype will be filtered. Multiple values may be specified by
using this argument more than once, and/or by providing a comma-separated list. Use
ValidateReference command to see the gene_biotypes in your GTF in order to decide what to exclude.
Default: not gene biotypes are filtered.
-d <dropseq_root> Directory containing Drop-seq executables. Default: directory containing this script.
-o <outputdir>
Where to write output bam. Default: current directory.
-t <tmpdir>
Where to write temporary files. Default: Value of 132153TMPDIR environment variable.
-a <STAR_path>
Full path of STAR. Default: STAR is found via PATH environment variable.
-b <bgzip_path>
Full path of bgzip: Default: bgzip is found via PATH environment variable.
-i <samtools_path>
Full path of samtools. Default: samtools is found via PATH environment variable.
-v verbose
-e merely echo commands instead of executing
SEE ALSO
Drop-seq_alignment.sh(1)
create_Drop-seq_reference_metadata.sh February 2021 CREATE_DROP-SEQ_REFERENCE_METADATA.SH(1)